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新聞中心

解析培養基各個成分的作用

發布時間:2022-04-18瀏覽次數:108返回列表


1.NaHCO3的作用是什么?


與二氧化碳一起形成緩沖系統,保持培養基PH值穩定。


2.丙酮酸鈉的作用是什么?


丙酮酸鈉可以作為細胞培養中的替代碳源,盡管細胞傾向于以葡萄糖作為碳源,但是如果沒有葡萄糖的話,細胞也可以代謝丙酮酸鈉。丙酮酸鈉的貯存液濃度一般為50mM,-20℃保存,培養基中的終濃度為1mM.


3.加入HEPES有何好處?


培養基中常用的Buffer system是碳酸氫鹽,它可提供營養,但卻在生理PH值條件下會降低緩沖能力。而HEPES是一種很強的Buffer,加入HEPES能使細胞培養基在PH7.2-7.6條件下緩沖能力增強。HEPES的貯存液濃度一般為1M,常溫保存。培養基中的終濃度為25mM,即5ml 1M的HEPES加入到200ml完全培養基中。


4.酚紅的作用是什么?


酚紅在培養基中被用來作為PH值指示劑:中性時為紅色,酸性時為黃色,堿性時為紫色。


5.谷氨酰胺的作用是什么?


幾乎所有的細胞對谷氨酰胺都有較高的要求。谷氨酰胺脫掉氨基后,可作為培養細胞的能量來源,參與蛋白質的合成和能量代謝。在缺少谷氨酰胺時,細胞生長不良而死亡。所以,各種培養液中都含有較大量的谷氨酰胺。

谷氨酰胺在溶液中很不穩定,應置-20℃冰凍保存,用前加入培養基中。加有谷氨酰胺的液體培養基4度冰箱儲存兩周以上時,應重新加入原來量的谷氨酰胺。

試驗操作中常配制高濃度(100倍)的谷氨酰胺溶液(1ml/支),分裝使用,-20℃保存。使用時,每支1ml的谷氨酰胺加入到99ml完全培養基中,使其終濃度為2mM培養基。


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